Journal: NPJ Biofilms and Microbiomes

Article Title: Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing

doi: 10.1038/s41522-024-00634-1

Figure Lengend Snippet: a Rarefactions curves (95% CI) of the number of observed species. Box and histogram plots for b Chao1, c Shannon index, and d Pielou indexes; e Rarified abundance of top 20 genera; f PCoA plot and explained variance for components 1 and 2 based on the Bray Curtis index. Results are based on rarefied sequences at a sequence depth of 17,520 reads.

Article Snippet: Fig. 3 Overview of ONT versus Illumina sequencing results for 407 saliva-extracted DNA samples at the phylum, genus, and species levels. a Venn diagrams illustrating numbers of shared taxa; b Scatter plots with regression line and 95% confidence interval of mean abundances; c Histograms with Kernel density lines for Spearman correlation coefficients of individual taxa.

Techniques: Sequencing

Journal: NPJ Biofilms and Microbiomes

Article Title: Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing

doi: 10.1038/s41522-024-00634-1

Figure Lengend Snippet: The inner circle shows a phylogenetic tree with color-marked phyla, and filled circles indicating significant phyla. The middle circle with boxes indicates relative abundance for each sample and subgroup. The bar graph on the outermost circle represents LDA coefficients exceeding 2.0 and differing significantly between sequence platforms (Wilcox test). All tests are adjusted for multiple comparisons (FDR).

Article Snippet: Fig. 3 Overview of ONT versus Illumina sequencing results for 407 saliva-extracted DNA samples at the phylum, genus, and species levels. a Venn diagrams illustrating numbers of shared taxa; b Scatter plots with regression line and 95% confidence interval of mean abundances; c Histograms with Kernel density lines for Spearman correlation coefficients of individual taxa.

Techniques: Sequencing

Journal: NPJ Biofilms and Microbiomes

Article Title: Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing

doi: 10.1038/s41522-024-00634-1

Figure Lengend Snippet: a Barplots of the observed number of species; b Shannon index; and c Bray Curtis distance PCoA plot (explained variance for components 1 and 2) based on serially diluted DNA samples from three clinical samples ranging from 800 ng to 6.5 ng. Results are generated at a sequencing depth of 49,365 reads.

Article Snippet: Fig. 3 Overview of ONT versus Illumina sequencing results for 407 saliva-extracted DNA samples at the phylum, genus, and species levels. a Venn diagrams illustrating numbers of shared taxa; b Scatter plots with regression line and 95% confidence interval of mean abundances; c Histograms with Kernel density lines for Spearman correlation coefficients of individual taxa.

Techniques: Generated, Sequencing